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TP53BP1 Antibody, Unconjugated, Rabbit, Polyclonal

TP53BP1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1257346

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Article Name:	TP53BP1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1257346
Supplier Catalog Number:	orb1257346
Alternative Catalog Number:	BYT-ORB1257346-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1763-1977 of human 53BP1 (NP_001135452.1).
Conjugation:	Unconjugated
Alternative Names:	TP53BP1, 53BP1, p202, p53-binding protein 1, p53-binding protein 53bp1, p53BP1

TP53BP1 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 255kDa
UniProt:	Q12888
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	TP53BP1

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of A-431 cells, using 53BP1 antibody (orb1257346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 40s.
	Immunohistochemistry of paraffin-embedded rat kidney using 53BP1 antibody (orb1257346) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using 53BP1 antibody (orb1257346) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using 53BP1 antibody (orb1257346) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using 53BP1 antibody (orb1257346). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using 53BP1 antibody (orb1257346). Green:GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

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