Western blot analysis of extracts of various cell lines, using RNF2 antibody (orb1257433) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using RNF2 antibody (orb1257433) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RNF2 antibody (orb1257433) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RNF2 antibody (orb1257433) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug RNF2 antibody (orb1257433). Western blot was performed from the immunoprecipitate using RNF2 antibody (orb1257433) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RNF2 antibody (orb1257433) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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