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EIF4B Antibody, Unconjugated, Rabbit, Polyclonal

EIF4B Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1257507

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Article Name:	EIF4B Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1257507
Supplier Catalog Number:	orb1257507
Alternative Catalog Number:	BYT-ORB1257507-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2).
Conjugation:	Unconjugated
Alternative Names:	EIF4B, EIF-4B, PRO1843

EIF4B Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 80kDa
UniProt:	P23588
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	EIF4B

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using EIF4B antibody (orb1257507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human liver cancer using EIF4B Antibody (orb1257507) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using EIF4B Antibody (orb1257507) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using EIF4B Antibody (orb1257507) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using EIF4B antibody (orb1257507). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug EIF4B antibody (orb1257507). Western blot was performed from the immunoprecipitate using EIF4B antibody (orb1257507) at a dilution of 1:1000.

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