HDGF Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1257542
Article Name: HDGF Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1257542
Supplier Catalog Number: orb1257542
Alternative Catalog Number: BYT-ORB1257542-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDGF (NP_004485.1).
Conjugation: Unconjugated
Alternative Names: Hepatoma-derived growth factor, HDGF, High mobility group protein 1-like 2, HMG-1L2, HDGF, HMG1L2
HDGF Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 38kDa
UniProt: P51858
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: HDGF
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HDGF antibody (orb1257542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using HDGF Antibody (orb1257542) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HDGF Antibody (orb1257542) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HDGF Antibody (orb1257542) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using HDGF antibody (orb1257542) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using HDGF antibody (orb1257542) at dilution of 1:100. Blue: DAPI for nuclear staining.