Western blot analysis of extracts of various cell lines, using NAA50 antibody (orb1257615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using NAA50 antibody (orb1257615) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NAA50 antibody (orb1257615) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using NAA50 antibody (orb1257615) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A549 cells using NAA50 antibody (orb1257615). Blue: DAPI for nuclear staining.
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