VARS Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1257740
Article Name: VARS Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1257740
Supplier Catalog Number: orb1257740
Alternative Catalog Number: BYT-ORB1257740-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human VARS (NP_006286.1).
Conjugation: Unconjugated
Alternative Names: VARS, G7A, VARS2, VARS1
VARS Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 140kDa
UniProt: P26640
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: VARS
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using VARS antibody (orb1257740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using VARS antibody (orb1257740) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using VARS antibody (orb1257740) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using VARS antibody (orb1257740) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using VARS Polyclonal Antibody (orb1257740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.