NELFE Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1257907
Article Name: NELFE Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1257907
Supplier Catalog Number: orb1257907
Alternative Catalog Number: BYT-ORB1257907-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3).
Conjugation: Unconjugated
Alternative Names: D6S45, NELF-E, RD, RDBP, RDP, negative elongation factor E, RD RNA-binding protein, RNA-binding protein RD, major histocompatibility complex gene RD, negative elongation factor polypeptide E, nuclear protein
NELFE Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 43kDa
UniProt: P18615
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: NELFE
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using NELFE antibody (orb1257907) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using NELFE antibody (orb1257907) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse Intestine using NELFE antibody (orb1257907) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NELFE antibody (orb1257907) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using NELFE antibody (orb1257907).