Western blot analysis of extracts of various cell lines, using NFkB p100/p52 antibody (orb1257951) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using NFkB p100/p52 antibody (orb1257951) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using NFkB p100/p52 antibody (orb1257951) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using NFkB p100/p52 antibody (orb1257951) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NFkB p100/p52 antibody (orb1257951) at dilution of 1:100. Blue: DAPI for nuclear staining.
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