AQP4 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258025
Article Name: AQP4 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258025
Supplier Catalog Number: orb1258025
Alternative Catalog Number: BYT-ORB1258025-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 244-323 of human Aquaporin 4 (NP_001641.1).
Conjugation: Unconjugated
Alternative Names: MIWC, WCH4, aquaporin-4, aquaporin type4, mercurial-insensitive water channel
AQP4 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 36kDa
UniProt: P55087
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: AQP4
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Aquaporin 4 antibody (orb1258025) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat lung using Aquaporin 4 antibody (orb1258025) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Aquaporin 4 antibody (orb1258025) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using Aquaporin 4 antibody (orb1258025) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of mouse brain using Aquaporin 4 Polyclonal Antibody (orb1258025) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.