TRAP1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258065
Article Name: TRAP1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258065
Supplier Catalog Number: orb1258065
Alternative Catalog Number: BYT-ORB1258065-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein of human TRAP1
Conjugation: Unconjugated
Alternative Names: TRAP1, HSP75, HSP90L, HSP 75, TRAP-1
TRAP1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 80kDa
UniProt: Q12931
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: TRAP1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using TRAP1 antibody (orb1258065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat skeletal muscle using TRAP1 Antibody (orb1258065) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TRAP1 Antibody (orb1258065) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TRAP1 Antibody (orb1258065) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TRAP1 antibody (orb1258065). Blue: DAPI for nuclear staining.