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E2F6 Antibody, Unconjugated, Rabbit, Polyclonal

E2F6 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258076

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Article Name:	E2F6 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1258076
Supplier Catalog Number:	orb1258076
Alternative Catalog Number:	BYT-ORB1258076-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
Conjugation:	Unconjugated
Alternative Names:	E2F6, E2F-6

E2F6 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 38kDa
UniProt:	O75461
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	E2F6

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

	Western blot analysis of extracts of MCF-7 cells, using E2F6 antibody (orb1258076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human esophageal cancer using E2F6 antibody (orb1258076) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using E2F6 antibody (orb1258076) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using E2F6 antibody (orb1258076). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells using 3 ug E2F6 antibody (orb1258076). Western blot was performed from the immunoprecipitate using E2F6 antibody (orb1258076) at a dilution of 1:500.

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