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COX5B Antibody, Unconjugated, Rabbit, Polyclonal

COX5B Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258089

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Article Name:	COX5B Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1258089
Supplier Catalog Number:	orb1258089
Alternative Catalog Number:	BYT-ORB1258089-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2).
Conjugation:	Unconjugated
Alternative Names:	Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide Vb, COX5B

COX5B Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 14kDa
UniProt:	P10606
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	COX5B

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100

	Western blot analysis of extracts of various cell lines, using COX5B antibody (orb1258089) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse heart using COX5B antibody (orb1258089) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using COX5B antibody (orb1258089) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using COX5B antibody (orb1258089) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using COX5B antibody (orb1258089) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of HepG2 cells, using 3 ug COX5B antibody (orb1258089). Western blot was performed from the immunoprecipitate using COX5B antibody (orb1258089) at a dilution of 1:1000.

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