H3K79me3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258179

Article Name:	H3K79me3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1258179
Supplier Catalog Number:	orb1258179
Alternative Catalog Number:	BYT-ORB1258179-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	ChIP, IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Other, Rat
Immunogen:	A synthetic methylated peptide corresponding to residues surrounding K79 of human histone H3
Conjugation:	Unconjugated
Alternative Names:	H3F3A, H3t, H3.4, H3/g, H3FT

H3K79me3 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 18kDa
UniProt:	Q16695
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	H3K79me3

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100

	Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K79 antibody (orb1258179). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K79 antibody (orb1258179) at 1:1000 dilution.
	Immunohistochemistry of paraffin-embedded human mammary cancer using TriMethyl-Histone H3-K79 antibody (orb1258179) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K79 antibody (orb1258179) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K79 antibody (orb1258179) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K79 antibody (orb1258179). Blue: DAPI for nuclear staining.
	Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H3K79me3 antibody (orb1258179) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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