H3K27me1 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
BYT-ORB1258187
- Images (7)
| Article Name: | H3K27me1 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | BYT-ORB1258187 |
| Supplier Catalog Number: | orb1258187 |
| Alternative Catalog Number: | BYT-ORB1258187-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, IF, IHC, IP, WB |
| Species Reactivity: | Human, Mouse, Other, Rat |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| Conjugation: | Unconjugated |
| Alternative Names: | H3F3A, H3t, H3.4, H3/g, H3FT |
| H3K27me1 Antibody |
| Clonality: | Polyclonal |
| Concentration: | batch dependent |
| Molecular Weight: | Observed: 18kDa |
| UniProt: | Q16695 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Form: | Liquid |
| Target: | H3K27me1 |
| Application Notes: | Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
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Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K27 antibody (orb1258187). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K27 antibody (orb1258187). |
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Immunohistochemistry of paraffin-embedded rat brain using MonoMethyl-Histone H3-K27 antibody (orb1258187) at dilution of 1:200 (40x lens). |
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Immunohistochemistry of paraffin-embedded human thyroid cancer using MonoMethyl-Histone H3-K27 antibody (orb1258187) at dilution of 1:200 (40x lens). |
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Immunohistochemistry of paraffin-embedded rat lung using MonoMethyl-Histone H3-K27 antibody (orb1258187) at dilution of 1:200 (40x lens). |
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Immunohistochemistry of paraffin-embedded human kidney cancer using MonoMethyl-Histone H3-K27 antibody (orb1258187) at dilution of 1:200 (40x lens). |
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Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K27 antibody (orb1258187). Blue: DAPI for nuclear staining. |
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