H3K4me3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258191

Article Name:	H3K4me3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1258191
Supplier Catalog Number:	orb1258191
Alternative Catalog Number:	BYT-ORB1258191-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	ChIP, IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Other, Rat
Immunogen:	A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
Conjugation:	Unconjugated
Alternative Names:	H3F3A, H3t, H3.4, H3/g, H3FT

H3K4me3 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 18kDa
UniProt:	Q16695
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	H3K4me3

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100

	Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K4 antibody (orb1258191). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody (orb1258191) at 1:1000 dilution.
	Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K4 antibody (orb1258191) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human rectal cancer using TriMethyl-Histone H3-K4 antibody (orb1258191) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K4 antibody (orb1258191). Blue: DAPI for nuclear staining.
	Chromatin immunoprecipitation analysis of extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody (orb1258191) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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