H3K4me2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258192
Article Name: H3K4me2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258192
Supplier Catalog Number: orb1258192
Alternative Catalog Number: BYT-ORB1258192-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: ChIP, IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Other, Rat
Immunogen: A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
Conjugation: Unconjugated
Alternative Names: H3F3A, H3t, H3.4, H3/g, H3FT
H3K4me2 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 18kDa
UniProt: Q16695
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: H3K4me2
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200CHIP seq: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody (orb1258192). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K4 antibody (orb1258192).
Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K4 antibody (orb1258192) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K4 antibody (orb1258192) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using DiMethyl-Histone H3-K4 antibody (orb1258192) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using DiMethyl-Histone H3-K4 antibody (orb1258192) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K4 antibody (orb1258192) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Lamin A/C antibody (orb1258730) at dilution of 1:100. Blue: DAPI for nuclear staining.