ITGB2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258240
Article Name: ITGB2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258240
Supplier Catalog Number: orb1258240
Alternative Catalog Number: BYT-ORB1258240-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 70-170 of human CD18 (NP_000202.3).
Conjugation: Unconjugated
Alternative Names: Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Complement receptor C3 subunit beta, CD18, ITGB2, CD18, MFI7
ITGB2 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 100kDa
UniProt: P05107
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: ITGB2
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CD18 antibody (orb1258240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded Rat lung using CD18 antibody (orb1258240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat spleen using CD18 antibody (orb1258240) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse spleen using CD18 antibody (orb1258240) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of RAW264.7 cells using CD18 antibody (orb1258240) at dilution of 1:100. Blue: DAPI for nuclear staining.