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EIF4E Antibody, Unconjugated, Rabbit, Polyclonal

EIF4E Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258245

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Article Name:	EIF4E Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1258245
Supplier Catalog Number:	orb1258245
Alternative Catalog Number:	BYT-ORB1258245-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF4E (NP_001959.1).
Conjugation:	Unconjugated
Alternative Names:	Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F

EIF4E Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 25kDa
UniProt:	P06730
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	EIF4E

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:50 - 1:100

	Western blot analysis of extracts of various cell lines, using EIF4E antibody (orb1258245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human lung cancer using EIF4E antibody (orb1258245) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using EIF4E antibody (orb1258245) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using EIF4E antibody (orb1258245) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug EIF4E antibody (orb1258245). Western blot was performed from the immunoprecipitate using EIF4E antibody (orb1258245) at a dilution of 1:1000.

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