SYK Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258274
Article Name: SYK Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258274
Supplier Catalog Number: orb1258274
Alternative Catalog Number: BYT-ORB1258274-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein of human SYK
Conjugation: Unconjugated
Alternative Names: SYK, spleen tyrosine kinase, HGNC:11491, p72-Syk
SYK Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 72KDA
UniProt: P43405
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: SYK
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using SYK antibody (orb1258274) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using SYK Antibody (orb1258274) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SYK Antibody (orb1258274) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SYK Antibody (orb1258274) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SYK antibody (orb1258274) at dilution of 1:100. Blue: DAPI for nuclear staining.