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PPP4C Antibody, Unconjugated, Rabbit, Polyclonal

PPP4C Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258283

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Article Name:	PPP4C Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1258283
Supplier Catalog Number:	orb1258283
Alternative Catalog Number:	BYT-ORB1258283-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1).
Conjugation:	Unconjugated
Alternative Names:	PPP4C, protein phosphatase 4 (formerly X), catalytic subunit, PP4, PPH3, PPX, Protein phosphatase 4, PP4C, PPP4

PPP4C Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 36kDa
UniProt:	P60510
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	PPP4C

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using PPP4C antibody (orb1258283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat liver using PPP4C antibody (orb1258283) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using PPP4C antibody (orb1258283) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using PPP4C antibody (orb1258283) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using PPP4C antibody (orb1258283). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug PPP4C antibody (orb1258283). Western blot was performed from the immunoprecipitate using PPP4C antibody (orb1258283) at a dilution of 1:1000.

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