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HDAC2 Antibody, Unconjugated, Rabbit, Polyclonal

HDAC2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258301

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Article Name:	HDAC2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1258301
Supplier Catalog Number:	orb1258301
Alternative Catalog Number:	BYT-ORB1258301-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Monkey, Mouse, Rat
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 390 to the C-terminus of human HDAC2 (NP_001518.3).
Conjugation:	Unconjugated
Alternative Names:	HDAC2 Antibody: HD2, RPD3, YAF1, Histone deacetylase 2, HD2

HDAC2 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 60kDa
UniProt:	Q92769
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	HDAC2

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using HDAC2 antibody (orb1258301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody (orb1258301) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody (orb1258301) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody (orb1258301) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using HDAC2 antibody (orb1258301). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of K562 cells using 1 ug HDAC2 antibody (orb1258301). Western blot was performed from the immunoprecipitate using HDAC2 antibody (orb1258301) at a dilution of 1:1000.

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