PDHA1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258402
Article Name: PDHA1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258402
Supplier Catalog Number: orb1258402
Alternative Catalog Number: BYT-ORB1258402-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1).
Conjugation: Unconjugated
Alternative Names: PDHA1, PDHA, PDHCE1A, PHE1A
PDHA1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 45kDa
UniProt: P08559
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: PDHA1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
Western blot analysis of extracts of various cell lines, using PDHA1 antibody (orb1258402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon carcinoma using PDHA1 antibody (orb1258402) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using PDHA1 antibody (orb1258402) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PDHA1 antibody (orb1258402) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using PDHA1 antibody (orb1258402) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using PDHA1 antibody (orb1258402) at dilution of 1:100. Blue: DAPI for nuclear staining.