DNMT1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258696
Article Name: DNMT1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258696
Supplier Catalog Number: orb1258696
Alternative Catalog Number: BYT-ORB1258696-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant fusion protein of Human DNMT1.
Conjugation: Unconjugated
Alternative Names: DNMT1, DNA (cytosine-5-)-methyltransferase 1, CXXC9, DNMT, FLJ16293, MCMT, MGC104992, DNA methyltransferase 1, AIM
DNMT1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 200kDa
UniProt: P26358
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: DNMT1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using DNMT1 antibody (orb1258696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using DNMT1 antibody (orb1258696) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DNMT1 antibody (orb1258696) at dilution of 1:100. Blue: DAPI for nuclear staining.