LMNB1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258832
Article Name: LMNB1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258832
Supplier Catalog Number: orb1258832
Alternative Catalog Number: BYT-ORB1258832-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide of human Lamin B1
Conjugation: Unconjugated
Alternative Names: LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419, OTTHUMP00000159218, lamin-B1
LMNB1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 75kDa
UniProt: P20700
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: LMNB1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (orb1258832) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast using Lamin B1 antibody (orb1258832) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Lamin B1 antibody (orb1258832) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Lamin B1 antibody (orb1258832) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Lamin B1 antibody (orb1258832) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (orb1258832) at dilution of 1:100. Blue: DAPI for nuclear staining.