DDX6 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1259076
Article Name: DDX6 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1259076
Supplier Catalog Number: orb1259076
Alternative Catalog Number: BYT-ORB1259076-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human DDX6 (NP_001244120.1).
Conjugation: Unconjugated
Alternative Names: DDX6, P54, RCK, HLR2
DDX6 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 54kDa
UniProt: P26196
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: DDX6
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using DDX6 antibody (orb1259076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using DDX6 Polyclonal Antibody (orb1259076) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.