VDAC1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1259361
Article Name: VDAC1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1259361
Supplier Catalog Number: orb1259361
Alternative Catalog Number: BYT-ORB1259361-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human VDAC1 / Porin (NP_003365.1).
Conjugation: Unconjugated
Alternative Names: VDAC1, PORIN, VDAC-1
VDAC1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 36kDa
UniProt: P21796
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: VDAC1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using VDAC1/Porin antibody (orb1259361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat heart using VDAC1/Porin antibody (orb1259361) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using VDAC1/Porin antibody (orb1259361) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using VDAC1/Porin antibody (orb1259361) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using VDAC1/Porin antibody (orb1259361) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using VDAC1/Porin antibody (orb1259361) at dilution of 1:100. Blue: DAPI for nuclear staining.