S100A8 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1259567
Article Name: S100A8 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1259567
Supplier Catalog Number: orb1259567
Alternative Catalog Number: BYT-ORB1259567-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human S100A8 (NP_002955.2).
Conjugation: Unconjugated
Alternative Names: 60B8AG, CAGA, calgranulin A, calgranulin-A, calprotectin L1L subunit, CFAG, CGLA, CP-10, cystic fibrosis antigen, L1Ag, leukocyte L1 complex light chain, MA387, MIF, migration inhibitory factor-related protein 8, MRP8, MRP-8, NIF, OTTHUMP00000015329, P8, protein S100-A8, S100 calcium binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, urinary stone protein band A
S100A8 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 11kDa
UniProt: P05109
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: S100A8
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using S100A8 antibody (orb1259567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human skin using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using S100A8 antibody (orb1259567) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of human skin cancer using S100A8 Polyclonal Antibody (orb1259567) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.