GLI1 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
BYT-ORB1259840
- Images (6)
| Article Name: | GLI1 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | BYT-ORB1259840 |
| Supplier Catalog Number: | orb1259840 |
| Alternative Catalog Number: | BYT-ORB1259840-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human GLI1 (NP_001153517.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | GLI1, GLI |
| GLI1 Antibody |
| Clonality: | Polyclonal |
| Concentration: | batch dependent |
| Molecular Weight: | Observed: 130kDa/160kDa |
| UniProt: | P08151 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Form: | Liquid |
| Target: | GLI1 |
| Application Notes: | Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
|
|
Western blot analysis of extracts of various cell lines, using GLI1 antibody (orb1259840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
|
|
Immunohistochemistry of paraffin-embedded rat testis using GLI1 antibody (orb1259840) at dilution of 1:100 (40x lens). |
|
|
Immunohistochemistry of paraffin-embedded human mammary cancer using GLI1 antibody (orb1259840) at dilution of 1:100 (40x lens). |
|
|
Immunohistochemistry of paraffin-embedded mouse testis using GLI1 antibody (orb1259840) at dilution of 1:100 (40x lens). |
|
|
Immunofluorescence analysis of HeLa cells using GLI1 antibody (orb1259840) at dilution of 1:100. Blue: DAPI for nuclear staining. |
|
|
Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody (orb1259840) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Product Guarantee and Expert Support
