COL1A1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1260380
Article Name: COL1A1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1260380
Supplier Catalog Number: orb1260380
Alternative Catalog Number: BYT-ORB1260380-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human COL1A1 (NP_000079.2).
Conjugation: Unconjugated
Alternative Names: Alpha-1 type I collagenOI4
COL1A1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 139kDa
UniProt: P02452
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: COL1A1
Application Notes: Application Notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using COL1A1 antibody (orb1260380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry of paraffin-embedded human colon carcinoma using COL1A1 antibody (orb1260380) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using COL1A1 antibody (orb1260380) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using COL1A1 antibody (orb1260380) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of rat ovary using COL1A1 antibody (orb1260380) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human breast cancer using COL1A1 antibody (orb1260380) at dilution of 1:100. Blue: DAPI for nuclear staining.