AK1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1260410
Article Name: AK1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1260410
Supplier Catalog Number: orb1260410
Alternative Catalog Number: BYT-ORB1260410-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1).
Conjugation: Unconjugated
Alternative Names: AK1, HTL-S-58j
AK1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 22kDa
UniProt: P00568
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: AK1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using AK1 antibody (orb1260410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using AK1 Antibody (orb1260410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using AK1 Antibody (orb1260410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using AK1 Antibody (orb1260410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using AK1 Antibody (orb1260410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using AK1 Antibody (orb1260410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using AK1 Antibody (orb1260410) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AK1 antibody (orb1260410). Blue: DAPI for nuclear staining.