CYCS Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1260421
Article Name: CYCS Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1260421
Supplier Catalog Number: orb1260421
Alternative Catalog Number: BYT-ORB1260421-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1).
Conjugation: Unconjugated
Alternative Names: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic
CYCS Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 14kDa
UniProt: P99999
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: CYCS
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Cytochrome c antibody (orb1260421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of HeLa cells using Cytochrome c antibody (orb1260421) at dilution of 1:100. Blue: DAPI for nuclear staining.