HAL Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1260634
Article Name: HAL Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1260634
Supplier Catalog Number: orb1260634
Alternative Catalog Number: BYT-ORB1260634-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HAL (NP_002099.1).
Conjugation: Unconjugated
Alternative Names: HAL, HIS, HSTD, histidase
HAL Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 73kDa
UniProt: P42357
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: HAL
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using HAL antibody (orb1260634) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using HAL antibody (orb1260634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HAL antibody (orb1260634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using HAL antibody (orb1260634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HAL antibody (orb1260634) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using HAL Polyclonal Antibody (orb1260634) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.