EP300 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1260637
Article Name: EP300 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1260637
Supplier Catalog Number: orb1260637
Alternative Catalog Number: BYT-ORB1260637-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EP300 (NP_001420.2).
Conjugation: Unconjugated
Alternative Names: EP300, E1A binding protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT, E1A-associated protein p300, E1A-binding protein, 300kD, p300, RSTS2
EP300 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 280kDa
UniProt: Q09472
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: EP300
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using EP300 antibody (orb1260637) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using EP300 antibody (orb1260637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EP300 antibody (orb1260637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using EP300 antibody (orb1260637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using EP300 antibody (orb1260637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EP300 antibody (orb1260637) at dilution of 1:100 (40x lens).