Western blot analysis of extracts of various cell lines, using EP300 antibody (orb1260637) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using EP300 antibody (orb1260637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EP300 antibody (orb1260637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using EP300 antibody (orb1260637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using EP300 antibody (orb1260637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EP300 antibody (orb1260637) at dilution of 1:100 (40x lens).
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