PGK2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1260661
Article Name: PGK2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1260661
Supplier Catalog Number: orb1260661
Alternative Catalog Number: BYT-ORB1260661-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2).
Conjugation: Unconjugated
Alternative Names: PGK2, PGK-2, PGKB, PGKPS, dJ417L20.2, HEL-S-272
PGK2 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 45kDa
UniProt: P07205
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: PGK2
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using PGK2 antibody (orb1260661) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human mammary cancer using PGK2 antibody (orb1260661) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using PGK2 antibody (orb1260661) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using PGK2 antibody (orb1260661) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using PGK2 Polyclonal Antibody (orb1260661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using PGK2 Polyclonal Antibody (orb1260661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.