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HDAC3 Antibody, Unconjugated, Rabbit, Polyclonal

HDAC3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1260861

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Article Name:	HDAC3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1260861
Supplier Catalog Number:	orb1260861
Alternative Catalog Number:	BYT-ORB1260861-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, WB
Species Reactivity:	Human, Mouse
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human HDAC3 (NP_003874.2).
Conjugation:	Unconjugated
Alternative Names:	HDAC3, RPD3, SMAP45, HD3, Histone deacetylase 3, RPD3-2

HDAC3 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 48kDa
UniProt:	O15379
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	HDAC3

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using HDAC3 antibody (orb1260861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
	Western blot analysis of extracts of mouse brain, using HDAC3 antibody (orb1260861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse liver using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using HDAC3 Polyclonal Antibody (orb1260861) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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