DDX3Y Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1261173
Article Name: DDX3Y Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1261173
Supplier Catalog Number: orb1261173
Alternative Catalog Number: BYT-ORB1261173-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1).
Conjugation: Unconjugated
Alternative Names: ATP-dependent RNA helicase DDX3Y, DEAD box protein 3, Y-chromosomal, DDX3Y, DBY
DDX3Y Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 73kDa
UniProt: O15523
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: DDX3Y
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using DDX3Y antibody (orb1261173) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat testis using DDX3Y antibody (orb1261173) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DDX3Y antibody (orb1261173) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX3Y antibody (orb1261173) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DDX3Y antibody (orb1261173) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using DDX3Y Polyclonal Antibody (orb1261173) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DDX3Y Polyclonal Antibody (orb1261173) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.