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PTK2 Antibody, Unconjugated, Rabbit, Polyclonal

PTK2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1261286

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Article Name:	PTK2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1261286
Supplier Catalog Number:	orb1261286
Alternative Catalog Number:	BYT-ORB1261286-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FAK (NP_722560.1).
Conjugation:	Unconjugated
Alternative Names:	PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FADK 1, Focal adhesion kinase 1, FAK1, p125FAK, PPP1R71, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, pp125 FA kinase, Pp125FAK

PTK2 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 120kDa
UniProt:	Q05397
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	PTK2

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

	Western blot analysis of extracts of various cell lines, using PTK2 antibody (orb1261286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human colon using FAK antibody (orb1261286) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using FAK antibody (orb1261286) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using FAK antibody (orb1261286) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC12 cells using FAK antibody (orb1261286) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of RAW264. 7 cells using FAK antibody (orb1261286) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug FAK antibody (orb1261286). Western blot was performed from the immunoprecipitate using FAK antibody (orb1261286) at a dilution of 1:1000.

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