Application Notes: TIGIT antibody can be used for ELISA starting at 0.1 µg/mLimmunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL. For flow cytometry at 1 µg/ml. For immunocytochemistry at 10µg/mL
Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10 µg/mL.
Immunofluorescence of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 20 µg/mL.
Immunofluorescence of TIGIT in human spleen tissue with TIGIT single domain antibody at 20 µg/mL.
Titration ELISA analysis of TIGIT sdAbs to detect recombinant TIGIT (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.
Immunohistochemistry of TIGIT in human spleen tissue with TIGIT single domain antibody at 1 µg/mL.
Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05 µg/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.
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