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POR Antibody, Unconjugated, Rabbit, Polyclonal

POR Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1274413

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Article Name:	POR Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1274413
Supplier Catalog Number:	orb1274413
Alternative Catalog Number:	BYT-ORB1274413-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POR (NP_000932.3).
Conjugation:	Unconjugated
Alternative Names:	POR, CPR, CYPOR, DKFZp686G04235, FLJ26468, P450R

POR Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 77kDa
UniProt:	P16435
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	POR

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using POR antibody (orb1274413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
	Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells, using POR antibody (orb1274413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human esophagus using POR antibody (orb1274413) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using POR Polyclonal Antibody (orb1274413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using POR Polyclonal Antibody (orb1274413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using POR Polyclonal Antibody (orb1274413) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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