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BECN1 Antibody, Unconjugated, Rabbit, Polyclonal

BECN1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1274427

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Article Name:	BECN1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1274427
Supplier Catalog Number:	orb1274427
Alternative Catalog Number:	BYT-ORB1274427-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
Conjugation:	Unconjugated
Alternative Names:	Beclin-1 Antibody: ATG6, VPS30, beclin1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein

BECN1 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 60kDa
UniProt:	Q14457
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	BECN1

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

	Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (orb1274427) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
	Western blot analysis of extracts from normal (control) and Beclin 1 knockout (KO) 293T cells, using Beclin 1 antibody (orb1274427) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human breast cancer using Beclin 1 antibody (orb1274427) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 antibody (orb1274427) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using Beclin 1 antibody (orb1274427) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug Beclin 1 antibody (orb1274427). Western blot was performed from the immunoprecipitate using Beclin 1 antibody (orb1274427) at a dilution of 1:1000.

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