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ELAVL1 Antibody, Unconjugated, Rabbit, Polyclonal

ELAVL1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1274439

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Article Name:	ELAVL1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1274439
Supplier Catalog Number:	orb1274439
Alternative Catalog Number:	BYT-ORB1274439-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, WB
Species Reactivity:	Human, Mouse
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR / ELAVL1 (NP_001410.2).
Conjugation:	Unconjugated
Alternative Names:	ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG

ELAVL1 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 36kDa
UniProt:	Q15717
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	ELAVL1

Application Notes:

Application Notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (orb1274439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
	Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (orb1274439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human esophagus using HuR/ELAVL1 antibody (orb1274439) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using HuR/ELAVL1 antibody (orb1274439) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using HuR/ELAVL1 antibody (orb1274439) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using HuR/ELAVL1 antibody (orb1274439) at dilution of 1:100. Blue: DAPI for nuclear staining.

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