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GNA13 Antibody, Unconjugated, Rabbit, Polyclonal

GNA13 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1274443

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Article Name:	GNA13 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1274443
Supplier Catalog Number:	orb1274443
Alternative Catalog Number:	BYT-ORB1274443-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant protein of human GNA13
Conjugation:	Unconjugated
Alternative Names:	Guanine nucleotide-binding protein subunit alpha-13, G alpha-13, G-protein subunit alpha-13, GNA13

GNA13 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 44kDa
UniProt:	Q14344
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	GNA13

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using GNA13 antibody (orb1274443) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and GNA13 knockout (KO) HeLa cells, using GNA13 antibody (orb1274443) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human stomach using GNA13 antibody (orb1274443) (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using GNA13 antibody (orb1274443) (40x lens).
	Immunofluorescence analysis of C6 cells using GNA13 Polyclonal Antibody (orb1274443) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using GNA13 Polyclonal Antibody (orb1274443) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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