S100A4 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1274487
Article Name: S100A4 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1274487
Supplier Catalog Number: orb1274487
Alternative Catalog Number: BYT-ORB1274487-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human S100A4 (NP_002952.1).
Conjugation: Unconjugated
Alternative Names: S100A4, S100 calcium binding protein A4, 18A2, 42A, CAPL, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium protein, malignant transformation suppression 1
S100A4 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 12kDa
UniProt: P26447
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: S100A4
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using S100A4 antibody (orb1274487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and S100A4 knockout (KO) HeLa cells, using S100A4 antibody (orb1274487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry of paraffin-embedded rat brain using S100A4 antibody (orb1274487) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using S100A4 antibody (orb1274487) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using S100A4 antibody (orb1274487) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using S100A4 antibody (orb1274487) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using S100A4 antibody (orb1274487) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of RAW264.7 cells using S100A4 antibody (orb1274487) at dilution of 1:100. Blue: DAPI for nuclear staining.