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EGFR Antibody, Unconjugated, Rabbit, Polyclonal

EGFR Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1274540

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Article Name:	EGFR Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1274540
Supplier Catalog Number:	orb1274540
Alternative Catalog Number:	BYT-ORB1274540-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Conjugation:	Unconjugated
Alternative Names:	Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

EGFR Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 175kDa
UniProt:	P00533
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	EGFR

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

	Western blot analysis of extracts of OVCAR3 cells, using EGFR antibody (orb1274540) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
	Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (orb1274540) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody (orb1274540) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody (orb1274540) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using EGFR antibody (orb1274540) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody (orb1274540) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC12 cells using EGFR antibody (orb1274540) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug EGFR antibody (orb1274540). Western blot was performed from the immunoprecipitate using EGFR antibody (orb1274540) at a dilution of 1:1000.

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