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SIRT1 Antibody, Unconjugated, Rabbit, Polyclonal

SIRT1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1274544

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Article Name:	SIRT1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1274544
Supplier Catalog Number:	orb1274544
Alternative Catalog Number:	BYT-ORB1274544-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2).
Conjugation:	Unconjugated
Alternative Names:	SIRT1 Antibody: SIR2L1, SIR2L1, NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, hSIRT1

SIRT1 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 140kDa
UniProt:	Q96EB6
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	SIRT1

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

	Western blot analysis of extracts of various cell lines, using SIRT1 antibody (orb1274544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) HeLa cells, using SIRT1 antibody (orb1274544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human mammary cancer using SIRT1 antibody (orb1274544) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using SIRT1 antibody (orb1274544) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using SIRT1 antibody (orb1274544) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug SIRT1 antibody (orb1274544). Western blot was performed from the immunoprecipitate using SIRT1 antibody (orb1274544) at a dilution of 1:1000.

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