Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human SPARC Antibody, the EC50 is 21.47 ng/ml.
Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human SPARC Antibody, the EC50 is 72.97 ng/ml.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (orb1291522) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (orb1291522) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Mouse Kidney tissue lysates, Lane 3: Mouse B16 cell lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-SPARC (orb1291522) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kDa, Observed band size: 33 kDa.
IHC-P analysis of rat placenta using SPARC Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of mouse kidney using SPARC Polyclonal Antibody at 1:200 dilution.
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