Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lowe
Target:
GPRC5D
Elisa plates were added with Flag Tag GPRC5D-Nanodisc (0.5 µg/per well) on an anti-Flag monoclonal antibody pre-coated (0.5 µg/per well) plate. Serial diluted anti-GPRC5D monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-GPRC5D monoclonal antibody binding with GPRC5D-Nanodisc is 32.86 ng/ml.
Human GPRC5D-Nanodisc, Flag Tag on SDS-PAGE
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