Claudin-1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB1294235
Article Name: Claudin-1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB1294235
Supplier Catalog Number: orb1294235
Alternative Catalog Number: BYT-ORB1294235-25,BYT-ORB1294235-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide
Conjugation: Unconjugated
Rabbit polyclonal antibody to Claudin-1
Clonality: Polyclonal
Buffer: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target: Claudin-1 / CLDN1
Application Dilute: Western Blot 1:1000 - 1:2000, Immunofluorescence 1:300 - 1:500
Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: Claudin 1 antibody (orb1294235).
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294235 anti-Claudin 1 antibody (red) at 1:400 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Immunohistochemical analysis of paraffin embedded Human prostate cancer tissue labeling Claudin-1 with orb1294235 at 1/100.
Lysates at 30 µg per lane. This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294235 overnight at 4C. anti-Claudin 1 antibody at 1/1000 dilution.