Synthetic peptide / encompassing a sequence within the C-terminus region.
Conjugation:
Unconjugated
Rabbit polyclonal antibody to COL1A1
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
COL1A1 / Collagen I
Application Dilute:
Western Blot 1:1000-1:1500, Immunofluorescence 1:300-1:500, Immunohistochemistry (Paraffin) 1:100-1:300
Anti-COL1A1 / Collagen I antibody - at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4C.
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min. Cells were stained with orb1294293 anti-COL1A1 / Collagen I antibody (red) at 1:400 and 4C.
COL1A1 / Collagen I antibody - at 1/1000 dilution. Lysates at 40 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4C.
COL1A1 / Collagen I antibody - at 1/250 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4C.
Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1 / Collagen I with orb1294293 at 1/150.
Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1 / Collagen I with orb1294293 at 1/150.
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1 / Collagen I with orb1294293 at 1/100.
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1 / Collagen I with orb1294293 at 1/100.
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