Synthetic peptide / Intracellular domain corresponding to Human EpCAM
Conjugation:
Unconjugated
Rabbit polyclonal antibody to EpCAM antibody (intracellular domain)
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
EpCAM / CD326 (Intracellular domain)
Application Dilute:
Western Blot 1:1000-1:2000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:200-1:300
All lanes: Anti-EpCAM antibody - at 1/800 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294312 overnight at 4C.
All lanes: Anti-EpCAM antibody - at 1/800 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294312 overnight at 4C.
Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294312 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294312 anti-EpCAM antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294312 at 1/200 RT 1hr.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294312 at 1/200 RT 1hr.
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